This Week In Microbiology

Mark Martin returns to TWiM for a discussion of the observation that Gram’s stain does not cross the bacterial cytoplasmic membrane, and suppression of gingival inflammation and bone loss through host modulation caused by episymbiotic Saccharibacteria.  Links for this episode Gram’s stain does not cross cytoplasmic membrane (ACS Chem Biol) Gram stain protocol (pdf) Chemical  mechanism of Gram’s stain (J Bact) Episymbiotic Saccharibacteria suppress disease (Host Cell Microbe) Microedu listserve TWiM Listener survey

Petra Levin joins TWiM to tell three stories from her laboratory: how starvation induces shrinkage of the bacterial cytoplasm; plasticity of E. coli cell wall and how it influences antibiotic resistance across different environments; and induction of antibiotic resistance by Triclosan. Hosts: Vincent Racaniello, Elio Schaechter, Michele Swanson, and Michael Schmidt Guest: Petra Levin Become a Patron of TWiM! Links for this episode Starvation induces E. coli shrinkage (PNAS) Plasticity of cell wall metabolism and antibiotic resistance (eLife) Triclosan induces antibiotic resistance (Antimicro Agents Chemother) TWiM Listener survey Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

On this episode of TWiM, using colicins to ferry DNA into cells through an iron transporter, and construction of highly efficient microbial fuel cells that produce more electrical current than previously observed. Links for this episode: Colicins used to ferry DNA into cells (mBio) Highly efficient microbial fuel cells (Science) Silver assists fuel cells (Science) Biological transport goes the extra mile (PNAS) Long distance transport in cable bacteria (PNAS) Columbia U pledges fossil free buildings (Columbia U) Become a patron of TWiM. Send your microbiology questions and comments to twim@microbe.tv

TWiM explores the role of biofilms in infection by coronaviruses, and development of a Shigella vaccine using outer membrane vesicles derived from Salmonella Become a patron of TWiM. Links for this episode Biofilms and coronaviruses (Appl Envir Micro) Outer membrane vesicle vaccine (Appl Envir Micro) TWiM Listener survey Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

On this episode, an electrochemical scaffold that delivers safe doses of hypochlorous acid to treat wound infections in humans, and a method for sampling and monitoring bacteria and viruses on surfaces using plain paper stickers. Hosts: Vincent Racaniello, Michele Swanson, and Michael Schmidt Links: E-scaffolds on TWiM 143 The EPS matrix (J Bact) Integrated HOCL-producing E-scaffold (AAM) Surface sampling bacteria with paper stickers (AEM) Surface sample viruses with paper stickers (Sci Rep) Become a Patron of TWiM! Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

TWiM reveals how temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts, and the intracellular localization of toxin-antitoxin proteins in E. coli. Become a Patron of TWiM! Links for this episode: Phage-pathogen conflicts (Science) A ‘Trap-Door’ Strategy for Mobile Element Escape (Front Micro) Location of toxin-antitoxin proteins (mBio) Babel-Jerusalem Bookstore Letters read on TWiM 249 Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

Mark Martin returns to TWiM to discuss ways to increase diversity in our field, and the discovery of Borgs, giant extrachromosomal elements with the potential to augment methane oxidation. Hosts: Vincent Racaniello, Elio Schaechter, Michele Swanson, and Michael Schmidt Guest: Mark O. Martin Subscribe to TWiM (free) on iTunes, Google Podcasts, Stitcher, Android, RSS, or by email.  Become a Patron of TWiM! Links for this episode: Unacknowledged privilege (Mol Biol Cell) Black Microbiologists Association Beginner’s Guide to Minority Professor Hires (ASM)Academic Career Readiness Assessment (UCSF) Annual Biomed Res Conference for Minority Students Lessons from Plants by Beronda Montgomery Giant extrachromosomal BORGS (bioRxiv) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

TWiM explores whether ‘rewilding’ is a way to get back our missing gut microbes, and failure of bacteriophage therapy due to the production of neutralizing antibodies. Become a patron of TWiM. Links for this episode Gut microbiota through an evolutionary lens (Science) You’re missing microbes (NY Times) There is no ‘healthy’ microbiome (NY Times) Antibody limits bacteriophage therapy (Nat) Phage therapy on TWiEVO #44 Graham Hatfull on TWiV #87 TWiM Listener survey Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

The TWiM folk explore disruption of a Burkholderia intracellular niche by a cell death program, and an increase in Brucella infectiousness after intracellular passage. Become a patron of TWiM. Links for this episode: Disrupting intracellular niche (mBio) Intracellular passage increases infectiousness (Infect Immun) Type IV secretion systems (Front Micro) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

In this episode, how polysaccharides keep cyanobacteria afloat in the oceans so that they can carry out photosynthesis, and a symbiotic bacterium that protects honey bees from fungal infections. Become a Patron of TWiM! Links for this episode: Bacterial blooms and polysaccharides (eLife) Social life of cyanobacteria (eLife) Bacteria that protect bees from fungi (mBio) First 21 days of a bee’s life (YouTube) antiSMASH (Nucl Acids Res) Delaney Miller’s website Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

Foodie TWiM reveals that bacteria in human saliva are major components of Ecuadorian indigenous beers, and an unusual E. coli that produces atypical light cream-colored colonies in chromogenic agar. Become a patron of TWiM. Links for this episode: Saliva bacteria in indigenous beers (Peer J) Unusual behavior of E. coli ST59 (Appl Envir Micro) One Health (CDC) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

TWiM continues its food arc with an examination of the effect of peroxyacetic acid spray on the microbiome and sensory properties of beef, and explores asymmetry of the cell division machinery during sporulation. Links for this episode: Effect of peroxyacetic acid on beef (Appl Envir Micro) Peracetic acid sterilization (CDC) Different cuts of beef (S. Clyde Weaver) Profiling the Poglianos (TWiM 115) Cell division machinery asymmetry in sporulation (eLife) Kamala Sohonie by Kanika Khanna India COVID SOS Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

TWiM reveals the microbiome of sourdough starter cultures, and discovery of a novel family of prokaryotic nanocompartments involved in the metabolism of sulfur. Become a patron of TWiM. Links for this episode Sourdough starter microbiomes (eLife) Prokaryotic nanocompartments (eLife) TWiM Listener survey Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

TWiM explains how Vibrio biofilms are dispersed by polyamine signals, and the induction of inappetence by respiratory virus infection which causes alteration of the gut microbiome. Subscribe to TWiM (free) on Apple Podcasts, Spotify, Google Podcasts, Android, RSS, or by email. Become a patron of TWiM. Links for this episode Regulation of Vibrio biofilms by polyamines (eLife) Cyclic di-GMP (pdf) Respiratory virus infection induces inappetance (mBio) Letters read on TWiM 241 TWiM Listener survey Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv  

TWiM reviews aspirin modulation of Fusobacterium nucleatum, a microbe that has been associated with colorectal cancer, and Elio tells us ‘What are vaccines’, a talk he recently gave to members of his community. Become a patron of TWiM. Links for this episode: Elio’s What are Vaccines? (pdf) Jenner Museum Aspirin modulation of Fusobacterium (mBio) Fecal transplant for C. difficile (NEJM) Fecal microbiota transplantation (NEJM) Fecal microbiota transplant for dysbiosis (NEJM) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

The TWiM team reviews how variants of P. aeruginosa survive antimicrobial treatment, and a decrease in the antimicrobial resistance of the gut microbiome in the presence of the fungus C. albicans. Become a patron of TWiM. Links for this episode: Phoenix colony variants of P. aeruginosa (AAC) Candida and the gut microbiota (mSphere) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

Elio reveals his thoughts on the big themes of modern microbiology, followed by an analysis of the gut microbiome in patients with Parkinson’s disease. Become a patron of TWiM. Links for this episode: Parkinson’s disease gut microbiome (NPJ Parkinsons) Sequencing data as compositions (Bioinformatics) Gut microbiota in mouse Parkinson’s model (Cell) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

To celebrate ten years, TWiM asks former hosts and guests to provide their thoughts on how microbiology has contributed to our understanding of the microbial world. Links for this episode: Neisseria LINES up: TWiM #1 Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

In this episode, hiring and training expectations for future biomedical life sciences faculty, and the roles of bacterial symbionts in deep-sea hydrothermal vent tubeworms. Become a patron of TWiM. Links for this episode: Expectations for life sciences faculty (Life Sci Edu) Academic career readiness assessment (UCSF) Tubeworm bacterial symbionts (eLife) Traveling on the Alvin (Dark Life II) Image credit Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

In this episode, how DNA of giant viruses has contributed extensively to the genome of green algae, and inhibition of E. coli virulence by a metabolic product of arachidonic acid in the intestinal epithelium. Links for this episode: Giant viral DNA shapes genomes of green algae (Nature) James Van Etten Darwin’s Radio Arachidonic acid and E. coli virulence (mBio) How a pathogenic E. coli infection begins Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv