This Week In Microbiology

TWiM reveals housefly dispersal of antimicrobial resistant bacteria, and a reproductive organ in squid linked to symbiotic bacteria. Links for this episode Housefly dispersal of antimicrobial resistant E. coli (Appl Micro Int) Antibiotic use in farming (Nature) Antimicrobial use in food producing animals (PLoS Glob Pub Health) Reproductive organ linked to symbiotic bacteria (mBio) Skype a Scientist Take the TWiM Listener survey!

TWiM reveals quorum-sensing systems that regulate intestinal inflammation and permeability caused by P. aeruginosa, and how plasmids manipulate bacterial behavior through translational regulatory crosstalk. Hosts: Vincent Racaniello, Michael Schmidt, Michele Swanson, Petra Levin. Become a patron of TWiM. Links for this episode Quorum-sensing in the intestine (mBio) Block quorum sensing, block biofilm (Antimicrob Agents Chemother) Plasmids manipulate bacterial behavior (PLoS Biol) Regulatory genes associated with integrative conjugative elements (J Bact) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv Music used on TWiM is composed and performed by Ronald Jenkees and used with permission.

TWiM presents a protocol for evolving caffeine-tolerant yeast by high school students in the home, and how predator-prey dynamics change when multiple bacteria grow together in biofilms. Hosts: Vincent Racaniello, Michael Schmidt, Michele Swanson, Petra Levin. Guest: Mark O. Martin Subscribe to TWiM (free) on Apple Podcasts, Spotify, Google Podcasts, Android, RSS, or by email. Become a patron of TWiM. Links for this episode Caffeine-tolerant yeast selected at home (microPub Biology) yEvo Lab Vision and change in undergraduate biology (NAS) CURE in a box (JMBE) EvolvingStem EvolvingStem: Evolution-in-action curriculum (BMC) Spatial ecology of predation (PNAS) Predatory bacteria: From curiosity to curative (Trends Micro) Combating antimicrobial resistance with predatory Bdellovibrio (YouTube) Bdellovibrio attacking E.coli (YouTube) From one, many (YouTube) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explains the synthesis in bacteria of new energy-dense biofuels that can replace rocket and jet fuels, and the use of nanopore sequencing to improve diagnosis and treatment of patients with serious infections.   Become a patron of TWiM.   Links for this episode:   Biosynthesis of high energy biofuels (Joule) Polyketide synthases in bacteria (PNAS) Sequencing for diagnosis of serious infections (mBio) Nanopore sequencing video (YouTube) Emerging human pathogen Kodamaea ohmeri (Front. Micro)

TWiM explains how magnesium modulates cell division frequency of a soil bacillus, and killing of fungi by Acinetobacter baumannii via a Type VI DNase Effector. Hosts: Vincent Racaniello, Michael Schmidt, Michele Swanson, and Petra Levin Subscribe to TWiM (free) on Apple Podcasts, Google Podcasts, Stitcher, Android, RSS, or by email. Become a Patron of TWiM! Links for this episode: •Magnesium modulates cell division frequency (J Bacteriol) •A. baumannii kills fungi (mBio) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

TWiM describes successful phage therapy against a mycobacterial lung infection, and how encapsulation of the cell wall protects S. pneumoniae from its major peptidoglycan hydrolase and host defenses. Links for this episode: Phage therapy against M. abscessus lung infection (Cell) Encapsulation of the septal cell wall protects S. pneumoniae from host defenses (PLoS Path) Letters read on TWiM 279

TWiM explores the relationship between the gut microbiome and depressive symptoms, and how purine nucleotides act as adjuvants to antibiotics. Hosts: Vincent Racaniello, Michael Schmidt, and Michele Swanson Subscribe to TWiM (free) on Apple Podcasts, Google Podcasts, Stitcher, Android, RSS, or by email. Become a Patron of TWiM! Links for this episode: Gut microbiota and depressive symptoms one and two (Nat Commun ) Microbiome influences depression (Phys.org) Gut bacteria and depression (Med Press) Mice behaving badly (TWiM 131) Gut microbiome in ASD (Front Cell Inf Micro) Transferring the blues (J Psych Res) Nucleotides as adjuvants to antibiotics (mBio) Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

On this episode of TWiM, we reveal widespread stop-codon recoding in bacteriophages that may regulate translation of lytic genes, and how Staphylococcus aureus inhibits Pseudomonas aeruginosa growth. Hosts: Vincent Racaniello, Michael Schmidt, and Michele Swanson. Become a patron of TWiM. Links for this episode Stop codon recoding in bacteriophages (Nat Micro) How S. aureus inhibits P. aeruginosa growth (J Bact) S. aureus small colony variants (Front Cell Infect Micro) Ken Timmis retires as journal editor (YouTube) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM presents evidence that over half of human pathogenic diseases are impacted by climate change, and considers how a novel prokaryote discovered next to an underground stream illuminates the pathway to multicellularity. Hosts: Vincent Racaniello, Michael Schmidt, Petra Levin and Mark Martin. Become a patron of TWiM. Links for this episode Climate change and pathogenic diseases (Nat Climate Change) Impacts of climate change on human diseases (MoraLAB) Cave bacteria illuminate pathway to multicellularity (eLife) Commentary on novel cave bacteria (eLife) Karst landscapes (National Park Service) Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM reveals high rates of co-transformation of plasmids in E. coli overturns the clonality myth, and bacterial membrane vesicles as a novel strategy for extrusion of the antimicrobial bismuth in H. pylori. Hosts: Vincent Racaniello, Michael Schmidt, and Petra Levin Links for this episode The myth of clonality (Sci Rep) Bacterial membrane vesicles extrude bismuth (mBio) Gastric acid levels must decrease (World J Gastroenterol) Take the TWiM Listener survey! Become a patron of TWiM. Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

Mark Martin returns to TWiM to join the discussion of how to design a complex gut microbiome, and protection of protists from virus infection by intracellular bacterial symbionts. Hosts: Vincent Racaniello, Michael Schmidt, Michele Swanson, and Petra Levin Become a patron of TWiM. Links for this episode Design of a complex gut microbiome (Cell) Defensive symbiosis against giant viruses (PNAS) Take the TWiM Listener survey! Music by Ronald Jenkees. Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM reveals how to inactivate norovirus on formica surfaces, and how to achieve antibiotic resistance by suppression of a frameshift mutation in an essential gene. Hosts: Vincent Racaniello, Michael Schmidt, Michele Swanson, and Petra Levin Subscribe to TWiM (free) on Apple Podcasts, Spotify, Google Podcasts, Android, RSS, or by email. Become a patron of TWiM. Links for this episode Effect of wiping on norovirus inactivation (Appl Env Micro) Chlorine dilution calculator (Ontario Pub Health) Antibiotic resistance by frameshift suppression (PNAS) Resistance to rifampicin (Nature) Take the TWiM Listener survey! Music by Ronald Jenkees. Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explores the activation of natural product synthesis using CRISPR interference in Streptomyces, and how light/dark and temperature cycling modulate Electron Flow in Pseudomonas aeruginosa biofilms. Hosts: Vincent Racaniello, Michele Swanson, and Petra Levin Become a patron of TWiM. Links for this episode Activating natural product synthesis (Nucleic Acids Res) Light and temperature modulate biofilm electron flow (mBio) Take the TWiM Listener survey! Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM presents a novel mucosal COVID-19 vaccine based on a bacteriophage capsid, and potentiation of C. difficile infection severity by the gut bacterial community. Become a patron of TWiM. Links for this episode: Bacteriophage based COVID-19 vaccine (mBio) Gut microbiome potentiates C. difficile disease (mBio) Letters read on TWiM 271 Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

TWiM explores the use of Archaea to produce plastics from molasses wastewater, and a bacterial defense against bacteriophage infection that involves depletion of deoxynucleotides. Become a patron of TWiM. Links for this episode Improving plastics production by Archaea (Appl Environ Micro) Biodegradability of PHA (Green Chem) What does tesla mean for an MRI and its magnet? (GE) Bacteria deplete nucleotides to block phages (Nat Micro) Antiphage hotspots in bacteria (TWiM 265) Take the TWiM Listener survey! Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM reviews discovery of a bacterium that is visible to the naked eye, and reversible resistance to bacteriophage by shedding of the bacterial cell wall. Become a patron of TWiM. Links for this episode A bacterium that is not a microbe (Science) Overwhelming microbial greatness (TWiM 261) Medical illustrator Kellie Holoski Bacteriophage resistance by shedding cell wall (Open Biol) Who came first, monderms or diderms? (Nat Ecol Evol) Letters read on TWiM 269 Take the TWiM Listener survey! Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM discusses citizen science surveillance of drug-resistant Aspergillus in garden soil, and the mechanism of action of a copper dependent antibiotic. Become a patron of TWiM. Links for this episode Fanny Hesse (Microbial Menagerie) Citizen science surveillance of Aspergillus (App Envir Micro) Aspergillum (Wikipedia) DMDC, copper dependent antibiotic (Infect Immun) National Summer Undergraduate Research Project Letters read on TWiM 268 Take the TWiM Listener survey! Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv

From ASM Microbe 2022 in Washington, DC, Heran joins TWiM to discuss her career and her work on the agent of tuberculosis, Mycobacterium tuberculosis. Hosts: Vincent Racaniello, Michael Schmidt, and Michele Swanson Guest: Heran Darwin Become a patron of TWiM. Links for this episode Honey badger of pathogens (EMBO Rep) Pup-proteasome system (PNAS) Cytokinin signaling in M. tuberculosis (mBio) Bandwagoning (EMBO Rep) Take the TWiM Listener survey! Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv

TWiM explains how spindle-shaped Archaeal viruses evolved from rod-shaped ancestors to package a larger genome, and transcriptional recording by CRISPR acquisition from RNA. Become a patron of TWiM. Links for this episode From rods to spindles (Cell) Spindle-shaped virus movie (Cell) Bacteria that record (Science) Transcriptional recording with CRISPR (Nature) Letters read on TWiM 266 TWiM Listener survey

TWiM explains the discovery of hotspots of genetic variation containing reservoirs of anti-phage systems in E. coli phages and their parasitic satellites, and pathogen desiccation tolerance promoted by hydrophilins. Become a patron of TWiM. Links for this episode Phage and satellite antiviral systems (Cell Host Micro) Hydropilins promote desiccation tolerance (Cell Host Micro) Letters read on TWiM 265 TWiM Listener survey Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments (email or recorded audio) to twim@microbe.tv