Histotalks: Nsh Podcasts

Title: Evaluating the Reverse Slide Embedding Method vs. Heat Extractor Embedding in the Mohs Laboratory: A Comparative Quality Review of 100 Cases Authors: Tashsa Cromedy, Heather Frye, Ochsner MD Anderson Cancer Center, St. Tammany Cancer Center A Campus of Ochsner Medical Center Abstract:  Overview Accurate tissue embedding is critical in Mohs micrographic surgery for complete margin assessment. This study evaluates the efficacy of a reverse slide embedding method compared to the conventional heat extractor technique. The goal was to determine which method yields fewer artifacts or discrepancies that may compromise histologic interpretation and margin assessment. Methods A total of 100 Mohs cases were retrospectively reviewed in a controlled laboratory setting. Two embedding techniques were compared: Reverse Slide Method: 50 cases were embedded by placing the tissue on a chilled slide before embedding, ensuring orientation preservation and minimizing heat exposure. Heat Extractor Method: 50 cases were embe