Histotalks: Nsh Podcasts

Novel Use of Pipette Based Capsules for Glycol Methacrylate, Methyl/Butyl Methacrylate and Epoxy Resin Processing with Mouse Tissue That Reduce Processing Solution Volume and Time.   Authors: Philip Seifert, HTL(ASCP), Xinyao Hu, Bianai Fan and Darlene A. Dartt, PhD, Schepens Eye Research Institute of Mass Eye and Ear, Boston, MA   The mPrep/S capsules (Microscopy Innovations) are customized pipette tips with screen inserts designed for various electron microscopy applications. Capsule processing methods were developed for mouse ocular and mineralized bone for histology and transmission electron microscopy (TEM) applications and compared to manual and carousel processed methods. The novel methods tested enable processing larger sized tissues (~3 mm diameter) including whole, anterior or posterior segments of mouse eyes and optic nerves within a single capsule that use 75% less reagents and time. Capsule processed tissue embedded in Glycol Methacrylate, methyl methacrylate/butyl methacrylate and EMBED812 epoxy